Generation of transcript counts from pasilla dataset with kallisto
نویسندگان
چکیده
The pasilla dataset was produced by Brooks et al. [1]. The aim of their study was to identify exons that are regulated by pasilla protein, the Drosophila melanogaster ortholog of mammalian NOVA1 and NOVA2 (well studied splicing factors). In their RNA-seq experiment, the libraries were prepared from 7 biologically independent samples: 4 control samples and 3 samples in which pasilla was knocked-down. The libraries were sequenced on the Illumina Genome Analyzer II using single-end and paired-end sequencing and different read lengths. The RNA-seq data can be downloaded from the NCBI’s Gene Expression Omnibus (GEO) under the accession number GSE18508.
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